Long-Read RNA Sequencing of the Purple Land Crab Gecarcoidea lalandii

by Ting, S.Y., Lau, N.-S., Sam, K.-K., Quah, E.S.H., Ahmad, A.B., Mat-Isa, M.-N., Shu-Chien, A.C.

Mr. Ting Seng Yeat, Dr. Lau Nyok Sean, Ms. Sam Ka Kei, Prof. Alexander Chong Shu Chien and collaborators Dr. Quah Evan S. H., Dr. Amirrudin B. Ahmad and Mr. Mohd-Noor Mat-Isa have published a research titled “Long-Read Sequencing Reveals the Repertoire of Long-Chain Polyunsaturated Fatty Acid Biosynthetic Genes in the Purple Land Crab, Gecarcoidea lalandii (H. Milne Edwards, 1837)” in Frontiers in Marine Science.

This paper reports on PacBio long-read RNA sequencing dataset of a land crab species, Gecarcoidea lalandii. A long-standing biological question in water to land transition is the capacity to adapt to an environment with lower supply of the physiologically important long chain polyunsaturated fatty acid (LC-PUFA) in land habitats. Accordingly, this study identified several transcripts related to the desaturation and elongation of polyunsaturated fatty acids, which may participate in the LC-PUFA biosynthesis pathway of a land crab. This will provide the impetus to functionally characterize these enzymes, providing the platform to understand the balance between dietary intake and in vivo biosynthesis of LC-PUFA during transition to terrestrial lifestyle. Importantly this yielded a novel gene catalog for a terrestrial crab species, which is an important addition to the current available brachyuran transcriptome datasets.



Link:  https://doi.org/10.3389/fmars.2021.713928

CCB Ref.:  2021_RH_2_lns
Date:  24/08/2021